Composite

Part:BBa_K1042015:Design

Designed by: Parth Patel   Group: iGEM13_Gaston_Day_School   (2013-09-26)


Cadmium Responsive GFP+Ogr Activator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 200
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1200


Design Notes

After creating two intermediate parts, the upstream one being the Cadmium Promoter with Ogr Activator (BBa_K1042012) and the downstream one being the PO Promoter with GFP (BBa_K1042013), we digested the intermediates and ligated them to the linearized pSB1C3 plasmid provided. The resulting colonies were tested for responsive GFP production from the addition of cadmium.

Source

Intermediates:

Original Parts:

  • Cadmium Promoter (BBa_K174015) registered by the Newcastle 2009 iGEM Team
  • Ogr Activator from P2 Phage (BBa_I746350) registered by the Cambridge 2007 iGEM Team
  • PO Promoter from P2 Phage (BBa_I746361) registered by the Cambridge 2007 iGEM Team
  • GFP with stops (BBa_I13401) registered by the MIT 2005 iGEM Team